What is SOC medium in transformation?
SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
What does SOC in SOC medium stand for?
Super Optimal broth
SOC Medium, shorthand for Super Optimal broth with Catabolite repression, is a nutritionally rich bacterial culture medium.
Can I use LB instead of SOC medium?
SOC is not mandatory, Instead you can also use LB broth. Transformation is depends on handling, heating-cooling and quantified DNA.
Why is SOC medium commonly used in transformation?
SOC Medium is a rich medium used primarily to aid recovery of bacterial competent cells following transformation. Use of SOC medium improves the molecular uptake whilst stabilizing the cells rapidly and so maximizing the efficiency of competent cells.
What does SOC medium contain?
S.O.C. Medium is supplied as 10 x 10 mL bottles of liquid medium with the following composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, and 20 mM glucose. Each lot of S.O.C. Medium is tested to ensure conformance with the most current approved product specifications.
How long does SOC medium last?
If stored properly, the medium should be stable for at least 1 year.
What is Luria broth made of?
LB Broth, also known as, LB medium, Lysogeny broth, Luria broth, or Luria-Bertani medium, is a commonly used nutritionally rich medium for culturing bacteria. First described in 1951 by Giuseppe Bertani, a 1-liter medium consists of 10 grams of tryptone, 5 grams of yeast extract, and 10 grams of sodium chloride.
How much DNA should I transform?
The volume of DNA to be added into competent cells does affect transformation efficiency. 1-5 µl of DNA (plasmid or ligation product) is recommended for 50 µl of competent cells. In 50 µl of competent cells, transformation efficiency drops to 52% when the DNA volume is increased to 10 µl (from 2 µl).
How do you make a SOB medium?
SOB medium is prepared without Mg++ by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 2 ml of 5M sodium chloride, and 2.5 ml of 1M KCl in water to a final volume of ≈ 1000 ml (980 ml).
Can you refreeze SOC media?
yes…i used to thaw FBS (fetal bovine serum), and thaw it whenever needed..it never affected my testings. Hi Amogh, I think its great to freeze up the media and use it whenever you can. But I recommend using it up within 2 weeks after freeze thawing.
Can you freeze SOC?
SOC medium is a bacterial growth medium used to maximize the transformation efficiency of cells, so it’s prone to bacterial contamination (it’s the glucose – the little guys love it!). Best to aliquot and freeze the medium.
What is the difference between Luria broth and nutrient broth?
So they are basically classified as complex media but both are non-selective as well, as numerous types of bacteria can grow in them and you cannot differentiate.
What is the composition of SOC medium?
Medium is supplied as 10 x 10 mL bottles of liquid medium with the following composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2, 10 mM MgSO 4, and 20 mM glucose. Each lot of S.O.C. Medium is tested to ensure conformance with the most current approved product specifications.
What is the recipe for SOC media?
What is the recipe for SOC medium? 1 Adjust to pH 7.0. 2 Autoclave and allow it to cool to temperature below 60°C. 3 Add 1 ml of filter-sterilized 2M Mg+2 (1M MgCl2 + 1M MgSO4). 4 Add 1 ml of filter-sterilized 2M glucose solution. 5 Add sterile dH2O to a final volume of 100 ml.
What is the composition of medium used in bacterial cell transformation?
Medium is used in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. S.O.C. Medium is supplied as 10 x 10 mL bottles of liquid medium with the following composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, and 20 mM glucose.
What is in SOC media for E coli?
In the SOC medium, tryptone and yeast extract provide E. coli with a source of carbon and nitrogen, in addition, the medium contains minerals, vitamins and amino acids. Sodium chloride and potassium chloride in the medium maintain ion transport and osmotic balance.