What is the difference between neurobasal A and neurobasal?
What is the difference between Gibco Neurobasal Medium and Gibco Neurobasal-A Medium? Neurobasal Medium is optimized for prenatal and fetal neurons. Neurobasal-A Medium is optimized for growing postnatal and adult brain neurons. These two media differ only in osmolality.
What is in Neurobasal medium?
NEUROBASAL Medium (1X) liquid
| Components | Molecular Weight | Concentration (mg/L) |
|---|---|---|
| Amino Acids | ||
| Magnesium Chloride (anhydrous) | 95.0 | 77.3 |
| Potassium Chloride (KCl) | 75.0 | 400.0 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 2200.0 |
How long does Neurobasal medium last?
Some cell lines may require an initial attachment in 2% serum‑supplemented complete Neurobasal™ Medium. Once supplemented, the complete Neurobasal™ Medium is stable for up to one week when stored in the dark at 2°C to 8°C. For Research Use Only. Not for use in diagnostic procedures.
What is Xeno free medium?
Description. Xeno-free and chemically-defined medium devoid of animal- or human-derived components. For stem cell expansion or other uses that must avoid variability due to undefined animal components.
What is N2B27 medium?
The N2B27 medium is a serum-free medium suitable for neural stem cell and pluripotent stem cell proliferation [26, 27].
What does b27 contain?
B-27 Serum-Free Supplement (50X) liquid
| Components | Concentration (mg/L) | mM |
|---|---|---|
| Vitamins | ||
| Glutathione (reduced) | confidential | n/a |
| L-Carnitine HCl | confidential | n/a |
| Linoleic Acid | confidential | n/a |
What is a basal medium?
1. Basal media. Basal media are those that can be used to grow (culture) bacteria without the need for additional media enrichment. Examples: Nutrient broth, nutrient agar and peptone water.
What is N2B27?
What is b27 supplement used for?
The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons.
What is animal origin free medium?
Animal Origin-Free* (AOF) Is not manufactured using any raw material ingredients derived from an animal (including human) tissue, cell, or body fluid (i.e. at the primary level of manufacturing); no animal-derived materials are used as a starting material or in the primary level manufacturing process.
What is Xeno-free culture?
It is my understanding that xeno-free is defined as all components (i.e. in the cell culture medium) are derived from the same organism whether it is animal (e.g. bovine) or human. These products could help us reach our goal of the ideal stem cell media – devoid of any animal or human derived components.
Is GlutaMAX the same as L glutamine?
Gibco GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture media. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.
What is the best medium to use for a glucose response study?
For neural studies that require the absence of insulin, glucose, and sodium pyruvate, such as glucose response or insulin response studies, we recommend Gibco B-27 Supplement Minus Insulin as a supplement to this medium. Neurobasal Medium is suitable for use in most neuronal cell applications.
Why GIBCO B-27 plus neuronal culture?
Increase cell survival by more than 50% with new Gibco™ B-27™ Plus Neuronal Culture System. Neurobasal-A medium without D-glucose and sodium pyruvate is used to support the growth of post-natal CNS neurons in applications where the presence of D-glucose and sodium pyruvate can cause interference.
Does a new neuromuscular medium support neurons in culture?
To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture.
Which extracellular basal media for single mature neurons?
The basic neuronal functions of single mature neurons were tested and compared in two classic extracellular basal media (DMEM/F12 or Neurobasal-A). The perfusion of extracellular media was switched from ACSF to DMEM/F12 or Neurobasal-A and back to ACSF for recovery.