How do you make a 1 kb DNA ladder?
If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).
How much does a DNA ladder cost?
1 kb DNA Ladder
| Catalog # | Concentration | List Price |
|---|---|---|
| N3232S | 500 µg/ml | $56.00 |
| N3232L | 500 µg/ml | $220.00 |
| Please enter a quantity for at least one size |
Where is the DNA ladder stored?
Quick-Load DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
How do you dilute a DNA ladder?
Preparation of DNA Ladder The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.
What is a 100 bp ladder?
Quick-Load® 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The digested DNA includes fragments ranging from 100-1,517 base pairs.
How do you make a 100 bp DNA Ladder?
This ladder is preferred by our lab because of its broad range (100 bp to 10,000 bp) and even intervals. Its stock concentration is 1 µg/µl of DNA. The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.
What is the recommended load for 1 KB plus DNA ladder?
Quick-Load 1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane. The following reagents are supplied with this product:
How much DNA ladder should I load per gel Lane?
We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane. In general, it is recommended to load 1µl per mm of gel lane. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
Can the quick-load DNA ladder be used to quantify DNA mass?
The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
How many KB is the DNA ladder on the Tae gel?
1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/gel lane. The following reagents are supplied with this product: