What is annexin V-FITC?
Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface. The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide.
What is annexin V assay?
The annexin V binding assay is a powerful tool for the quantification of apoptosis and for distinguishing between apoptotic and necrotic cells and is used by many laboratories.
What is annexin V flow cytometry?
Annexin-V is a specific PS-binding protein that can be used to detect apoptotic cells. The Millipore Muse mini flow cytometer can be used to determine apoptosis levels in a cell sample by labelling with annexin V-PE and 7-AAD as the viability dye, see figure.
Is Annexin V an antibody?
Anti-annexin V antibodies (aANVAs) are detected in various diseases like rheumatoid arthritis, systemic lupus erythematosus and anti-phospholipid antibody syndrome. The study of ANV in Acute Myocardial Infarction (AMI) might shed light on hypercoagulability mechanisms in the pathogenesis of acute coronary syndromes.
What is Annexin V binding buffer?
The Annexin V binding buffer is formulated for flow cytometric staining of apoptotic cells with conjugated Annexin V. The buffer is provided ready to use.
What is Annexin V binding?
Annexin V binds is a phospholipid phosphatidylserine (PS) binding protein. This can be used to distinguish cells that are viable (Annexin V and 7-AAD negative), early apoptotic (Annexin V positive and 7-AAD negative), and those that are dead or in late apoptosis (Annexin V and 7-AAD positive).
What Colour is annexin V?
green
Cells that have bound annexin V-FITC will show green staining in the plasma membrane. Cells that have lost membrane integrity will show red staining (PI) throughout the nucleus and a halo of green staining (FITC) on the cell surface (plasma membrane).
What does annexin V positive mean?
Annexin V positive – PI positive staining indicate cells are in necrosis (post-apoptotic necrosis or late apoptosis).
Can you fix Annexin V?
In my experience, it is possible to fix annexin V stained cells and see great results on the flow. Stain cells with annexin V (in calcium-rich binding buffer). Wash cells in binding buffer to remove unbound AxV. Fix in your choice of fixative (diluted in binding buffer).
What is the purpose of binding buffer?
DNA Binding Buffer is designed for use in the DNA Clean & Concentrator family of products. This buffer facilities efficient binding and recovery of DNA from enzymatic reactions and impure DNA samples.
Is annexin V an antibody?
What is the concentration of annexin V-FITC in the TACS assay?
TACS Annexin V-FITC is provided at a 100X concentration with a 1:1 molar ratio of Annexin V to FITC. The binding of Annexin V will vary depending upon cell type and assay method, therefore the concentration of Annexin V used may require optimization.
What is annexin V-FITC apoptosis detection kit?
The TACS™ Annexin V-FITC Apoptosis Detection Kit facilitates rapid fluorometric quantitation of apoptotic cells either by flow cytometry or in situdetection. Annexin V Ca2+ Ca2+Ca 2+ Ca2+ Ca2+ Apoptosis Inner Cell Membrane Outer Cell Membrane Phosphatidylserine Other Phospholipids Annexin V
Can TACS™ annexin V-FITC and propidium iodide cells be viewed together?
Cells may be viewed through a dual pass filter allowing you to visualize the TACS™ Annexin V-FITC positive and the Propidium Iodide positive cells in the same field, however, there may be significant signal overlap between FITC and Propidium Iodide making the interpretation of results difficult.
What is annexin V used for?
Annexin V allows for the identification of cell surface changes that occur early during the apoptotic process. The TACS™ Annexin V-FITC Apoptosis Detection Kit facilitates rapid fluorometric quantitation of apoptotic cells either by flow cytometry or in situdetection.