Which medium is used to culture BHK cells?
The medium is a 1:1 (v/v) mixture of Dulbecco’s modified Eagle’s medium and Ham’s F-12 medium supplemented with fibroblast growth factor, fibronectin, insulin, oleic acid (preincubated with fatty-acid-free bovine serum albumin as carrier), and transferrin.
What is BHK cell line?
Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.
Why are BHK cells used?
18.5. Baby hamster kidney (BHK) cells are currently used for vaccine production as well as production of a few recombinant proteins such as Factor VIIa (NovoSeven®) and FVIII (Kogenate®).
What is the doubling time of BHK cells?
BHK-21 cells grew with a doubling time of 12 h, while BHKRJ cells had a 24-h lag, followed by growth at a rate of 13 h per doubling.
Can I use DMEM instead of emem?
Moreover, DMEM contains four times more vitamins and amino acids in comparison to EMEM. Similarly, it contains two or four times more glucose in comparison to EMEM. Also, the optimal glucose concentration for many types of cells is 4500 mg/L in DMEM. Thus, it makes DMEM is more suitable for specific cell types.
Can virus be cultured in synthetic medium?
A specialized culture medium containing the extract of plant and animal tissues is used for the growth of viruses. Viruses are nonliving organisms that get active when they are in contact with the host cell. No virus can be grown on an artificial culture medium as there is no living cell present.
What is a cell line in cell culture?
Cell Line. A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. Lines differ from cell strains in that they become immortalized.
What is the role of DMEM in cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Which bacteria Cannot grow in artificial media?
Therefore, it has been clear from the above discussion that Mycobacterium leprae is a bacterium that cannot be grown on synthetic media.
How are BHK-21 cells harvested and stored?
After several days, the infected cells are harvested, lysed by repeated freezing and thawing, sonicated, dispensed in small aliquots, and stored at − 80°C. NOTE: BHK-21 cells should be acquired from ATCC, as cells from alternative sources may support lower levels of MVA replication.
What is BHK-21 (C-13)?
This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII, see ATCC CRL-8544 ). The parent line of BHK-21 (C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker.
What does ATCC mean for living cultures?
For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product.
How do I get a certificate of analysis for BHK-21 [C-13] (ccl-10)?
To download a certificate of analysis for BHK-21 [C-13] ( CCL-10 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of BHK-21 [C-13] ( CCL-10) is not currently available online. Complete this form to request this certificate of analysis.